SILAM refers to labeling an entire rodent with heavy stable isotopes for quantitative proteomic tissue analysis. Labeling an entire proteome with heavy isotopes in vivo generates an ideal standard for quantitative proteomics.
When a heavy proteomics labeled proteome is mixed with an unlabeled proteome then digested, every unlabeled peptide identified by the mass spectrometer can be quantified by its corresponding heavy peptide. The advantage of metabolic labeling over in vitro tagging techniques is that the heavy and unlabeled samples are mixed before sample preparation, preventing variability between preparations from distorting the quantification results. This is especially important when extensive sample preparation (e.g. isolation of an organelle) is required.
In SILAM, the rodent food is altered to contain heavy lysine or 15N spirulina as the only protein source. The heavy tissues are used as internal standards for quantitative proteomic analysis of basic mammalian physiology and animal models of disease. We are also pleased to offer intact stable isotope-enriched mouse tissue for use in SILAM: Mouse Express® L-Lysine (13C6, 97%) Mouse Tissue and Mouse Express® (15N, 94%) Mouse Tissue from Cambridge Isotope Laboratories, Inc. (CIL).
Mouse Express® is a registered trademark of Cambridge Isotope Laboratories, Inc.