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Cell Free Sciences Special discount

UP TO 30% OFF until December 31, 2021 With the promo code CELLFREE2021

 

CellFree Sciences is proud to offer a high-performance wheat germ cell-free protein expression system covering a wide range of needs in protein research. Depending on the product, reagents are commonly shipped on dry ice using an express carrier.

Working outside the cell, in vitro protein expression systems can be modified to your experimental needs. One of the most well-established method is the wheat germ cell-free protein synthesis system offered by CellFree Sciences. It extends on what is possible using other small-scale in vitro translation kits on the market and gives the users more opportunities to work on different reaction scales. Using their Core Kit format, protein expression reactions can yield up to 1 mg of crude protein (yield given for GFP reference template) depending on the reaction size used.

CellFree Sciences’ Core Kits are available in different formats demonstrating the ability to adjust reaction conditions in cell-free systems: In their G and H Versions, the wheat germ extracts are pretreated on a glutathione- or Ni resin to remove wheat proteins that could otherwise bind to those resins and copurify together with tagged proteins (see example). Thus, these kits allow for a higher protein purity when working with the common GST- or His-tag in a one-step purification or using the GST-tag to capture proteins for example in binding assays.

Moreover, CellFree Sciences offers their Core Kits in amino acid free versions to support labeling experiments for example to prepare standards for protein mass spectrometry1,2. These versions of the wheat germ cell-free system are further one of the most proven approaches for preparing samples for protein NMR as recently reviewed3. Similarly, proteins made in the wheat germ system have been studied by other methods to determine their structures as described in more details in the aforementioned review that also provides guidance on how to use the expression system effectively.

Use our special offer to purchase a Core Kit from CellFree Sciences and try their wheat germ cell-free protein expression system for your protein needs. We will add a free vector set to each order received during the campaign!

Comparing protein purity working with the GST- and His-tag in combination with the special G and H versions of the WEPRO7240 wheat germ extract: 1: Human-derived protein fused to GST tag; 2: Human-derived protein fused to His tag. N: Negative control without added template (proteins in wheat germ extract); S: purified protein regular WEPRO7240 wheat germ extract; G: purified protein pretreated WEPRO7240G wheat germ extract; H: purified protein pretreated WEPRO7240H wheat germ extract. Background binding indicated by * (glutathione binding protein); * (Ni binding proteins).

 

References:

  1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5724179/ (open access)
  2. https://link.springer.com/protocol/10.1007%2F978-1-61779-885-6_19
  3. https://www.frontiersin.org/articles/10.3389/fmolb.2021.639587/full (open access)

Linked cell-free protein expression reactions for protein research applications (Bilayer Format)
Premixed reagents for linked cell-free transcription and translation reaction on 226 μl
Item number Product Name Description Reaction Web Price New Price
CFS-C7 WEPRO7240 Core Kit SMALL reaction scale of 227 μl (up to 100 reactions), a MEDIUM reaction scale of 1.2 ml (up to 20 reactions), and a LARGE reaction scale of 6 ml (up to 4 reactions). 1 ml extract 1 496 € 1 047,20 €
CFS-C7G WEPRO7240G Core Kit SMALL reaction scale of 227 μl (up to 100 reactions), a MEDIUM reaction scale of 1.2 ml (up to 20 reactions), and a LARGE reaction scale of 6 ml (up to 4 reactions). Kit uses precleared extract indented for preparing GST-fusion proteins. 1 ml extract 1 224 € 1 142,40 €
CFS-C7H WEPRO7240H Core Kit SMALL reaction scale of 227 μl (up to 100 reactions), a MEDIUM reaction scale of 1.2 ml (up to 20 reactions), and a LARGE reaction scale of 6 ml (up to 4 reactions). Kit uses precleared extract indented for preparing His-tagged fusion proteins. 1 ml extract 1 632 € 1 142,40 €
CFS-C8 WEPRO8240 Core Kit WEPRO8240 is WEPRO7240 without amino acids to enable protein labeling reactions. SMALL reaction scale of 240 μl (up to 100 reactions), a MEDIUM reaction scale of 1.2 ml (up to 20 reactions), and a LARGE reaction scale of 6 ml (up to 4 reactions). 1 ml extract 1 496 € 1 047,20 €
CFS-C8G WEPRO8240G Core Kit WEPRO8240G is WEPRO7240G without amino acids to enable protein labeling reactions. SMALL reaction scale of 240 μl (up to 100 reactions), a MEDIUM reaction scale of 1.2 ml (up to 20 reactions), and a LARGE reaction scale of 6 ml (up to 4 reactions). Kit uses precleared extract indented for preparing GST-fusion proteins. 1 ml extract  1 632 € 1 142,40 €
CFS-C8H WEPRO8240H Core Kit WEPRO8240H is WEPRO7240H without amino acids to enable protein labeling reactions. SMALL reaction scale of 240 μl (up to 100 reactions), a MEDIUM reaction scale of 1.2 ml (up to 20 reactions), and a LARGE reaction scale of 6 ml (up to 4 reactions). Kit uses precleared extract indented for preparing His-tagged fusion proteins. 1 ml extract     1 632 € 1 142,40 €

Special applications
Protein labeling for protein mass spectrometry
Item number Product Name Description Reaction Web Price New Price
CFS-FLEX-MS FLEXIQuant PLUS Expression Kit Protein synthesis kit with 13C/15N labeled lysine and arginine for MRM/SRM mass spectrometry. Kit uses bilayer reaction on 226 μl scale. This kit provided expression vector and peptide to conduct FLEXIQuant experiments for using the FLEX-tag for quantifying the reference standard.  8  1 088 € 761,60 €
CFS-PLUS-MS Premium PLUS Expression Kit for MS

Protein synthesis kit with 13C/15N labeled lysine and arginine for MRM/SRM mass spectrometry. Kit uses bilayer reaction on 226 μl scale. This kit contains an expression vector, a positive control, and a PCR primer set.
8 vials 1 632 € 1 142,40€
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