Chemical Tagging

Metabolic incorporation of heavy isotopes into a proteome, as in SILAC and SILAM, is a popular method to prepare an internal standard or labeled control; however, some organisms and animals are not amenable to metabolic incorporation. Fortunately, analytes may be readily modified through chemical tagging reactions. Examples include the reductive animation of primary amines in proteins or peptides and hydrazide tagging of free N-linked glycans in proteomic samples.

Since tagging reagents are compatible with many biological sample types, we are delighted to offer a collection of reductive methylation reagents and a glycan-tagging kit termed INLIGHT®(Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tag) for 2- or 3-plex relative quantitation.


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