Metabolic Labelling

Stable isotope amino acids metabolically incorporated for quantitative proteomics

One type of proteome labeling introduces a stable isotope amino acid(s) to cell growth media or rodent feed. Growth and feeding periods allow the stable isotope-labeled amino acid(s) to become metabolically incorporated into the proteome. Experiments involving cell culture are referred to as SILAC (stable isotope labeling by amino acids in cell culture), while mammalian systems are referred to as SILAM (stable isotope labeling in mammals).