INLIGHT® Kit
A glycan-tagging kit for relative quantification by mass spectrometry
Glycans participate in a large number of cellular, molecular, and biological processes and are implicated in a number of diseases (e.g., Alzheimer's, cancer). To aid the identification and relative quantification of glycans by LC-MS, Eurisotop offers the innovative INLIGHT® (Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tag) glycan-tagging kit. This kit employs both natural (NAT) and stable isotope-labeled (SIL; 13C6) phenyl 2-GPN reagents in the hydrazide derivatization of free glycans. While this tagging strategy was developed for N-glycans, it has also been adapted to O-glycan and heparin oligomer profiles.
INLIGHT® was developed by David Muddiman and colleagues at North Carolina State University. The phenyl 2-GPN reagent offers significant advantages over other tagging strategies, including rapid and efficient derivatization and enhanced hydrophobicity and ionization efficiency — facilitating the relative quantification of typically polar glycans by reversed-phase LC-MS.
Kit contents
5 vials NAT reagent
Natural (light) phenyl 2-GPN
5 vials SIL reagent
13C6-labeled (heavy) phenyl 2-GPN
User manual
Step-by-step protocol with maltoheptaose & fetuin A examples
relative quantification experiments per kit
